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首页> 外文OA文献 >Pyruvate dehydrogenase kinase activity of pig heart pyruvate dehydrogenase (E1 component of pyruvate dehydrogenase complex).
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Pyruvate dehydrogenase kinase activity of pig heart pyruvate dehydrogenase (E1 component of pyruvate dehydrogenase complex).

机译：猪心脏丙酮酸脱氢酶（丙酮酸脱氢酶复合体的E1组分）的丙酮酸脱氢酶激酶活性。

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摘要

The pyruvate dehydrogenase (E1) and acetyltransferase (E2) components of pig heart and ox kidney pyruvate dehydrogenase (PDH) complex were separated and purified. The E1 component was phosphorylated (alpha-chain) and inactivated by MgATP. Phosphorylation was mainly confined to site 1. Addition of E2 accelerated phosphorylation of all three sites in E1 alpha and inactivation of E1. On the basis of histone H1 phosphorylation, E2 is presumed to contain PDH kinase, which was removed (greater than 98%) by treatment with p-hydroxymercuriphenylsulphonate. Stimulation of ATP-dependent inactivation of E1 by E2 was independent of histone H1 kinase activity of E2. The effect of E2 is attributed to conformational change(s) induced in E1 and/or E1-associated PDH kinase. PDH kinase activity associated with E1 could not be separated from it be gel filtration or DEAE-cellulose chromatography. Subunits of PDH kinase were not detected on sodium dodecyl sulphate/polyacrylamide gels of E1 or E2, presumably because of low concentration. The activity of pig heart PDH complex was increased by E2, but not by E1, indicating that E2 is rate-limiting in the holocomplex reaction. ATP-dependent inactivation of PDH complex was accelerated by E1 or by phosphorylated E1 plus associated PDH kinase, but not by E2 plus presumed PDH kinase. It is suggested that a substantial proportion of PDH kinase may accompany E1 when PDH complex is dissociated into its component enzymes. The possibility that E1 may possess intrinsic PDH kinase activity is considered unlikely, but may not have been fully excluded.
机译：分离并纯化猪心和牛肾脏丙酮酸脱氢酶（PDH）复合物的丙酮酸脱氢酶（E1）和乙酰基转移酶（E2）。 E1组分被磷酸化（α链）并被MgATP灭活。磷酸化主要限于位点1。E2的添加加速了E1α中所有三个位点的磷酸化和E1的失活。根据组蛋白H1的磷酸化，推测E2包含PDH激酶，通过用对羟基巯基苯基磺酸盐处理可将其去除（大于98％）。 E2对E1的ATP依赖性失活的刺激与E2的组蛋白H1激酶活性无关。 E2的作用归因于在E1和/或与E1相关的PDH激酶中诱导的构象变化。与E1相关的PDH激酶活性无法通过凝胶过滤或DEAE-纤维素色谱法分离出来。在E1或E2的十二烷基硫酸钠/聚丙烯酰胺凝胶上未检测到PDH激酶的亚基，可能是因为浓度低。猪心脏PDH复合物的活性通过E2增加，但没有通过E1增加，表明E2在全络合物反应中是限速的。 E1或磷酸化的E1加相关的PDH激酶可加速PDH复合物的ATP依赖性失活，但E2加假定的PDH激酶则不会。提示当PDH复合物解离成其组成酶时，大部分的PDH激酶可能伴随着E1。 E1可能具有固有的PDH激酶活性的可能性被认为是不可能的，但可能尚未被完全排除。

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    Kerbey, A L; Randle, P J;

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  • 年度 1985
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